Movie 2.
Localisation of endocytosed HSA-AF488 to LysoTracker Red-positive compartments in live BOECs. Cultured BOECs were stained with LysoTracker Red DND-99 (red) for 60 minutes at 37°C and subsequently pulsed with HSA-AF488, green for 15 minutes at 37°C and then chased. Live cells were imaged at 37°C and 5% CO2 using an Olympus FV3000 confocal fluorescence microscope from 30 minutes chase with a 20 second delay between image capture.
The endosomal system of primary human vascular endothelial cells and albumin–FcRn trafficking
J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.260912