Movie 3.
Intracellular trafficking of HSA-AF488 in FcRn-mCherry transduced live BOECs. BOECs transduced with FcRn-mCherry (red) were pulsed with HSA-AF488 (green) for 15 minutes at 37°C and the fluorescent signal chased for 40 minutes in live cells and live BOECs were imaged at 37°C and 5% CO2 with a FV3000 Olympus confocal microscope. The video starts at 10 minutes of chase with a 20 second delay between image capture.
The endosomal system of primary human vascular endothelial cells and albumin–FcRn trafficking
J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.260912