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Movie 3.

Intracellular trafficking of HSA-AF488 in FcRn-mCherry transduced live BOECs. BOECs transduced with FcRn-mCherry (red) were pulsed with HSA-AF488 (green) for 15 minutes at 37°C and the fluorescent signal chased for 40 minutes in live cells and live BOECs were imaged at 37°C and 5% CO2 with a FV3000 Olympus confocal microscope. The video starts at 10 minutes of chase with a 20 second delay between image capture.

The endosomal system of primary human vascular endothelial cells and albumin–FcRn trafficking

Andreas Pannek, Janine Becker-Gotot, Steven K. Dower, Anne M. Verhagen, and Paul A. Gleeson

J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.260912