Movie 5.

RGC growth cone filopodium expressing mNG-TOCA-1 and mScarlet-Ena, showing that the two proteins coincide during filopodial protrusion events (I = initiation, M = re-extension after merging, L = re-extension after lamellipodium catching up with filopodium). 3 minute video acquired at 1.5 s per frame, replayed at 10 frames per second.