Movie 5.
Time-lapse Airyscan confocal movie related to Fig. S6A. Images were acquired using Airyscan fast mode as 12 z-stacks every 5 min. Images were processed using “3D Airyscan processing” using ZEN Black software and max projected. White arrow indicates the interaction between GFP-SEPT6 and mCherry-LC3B mediating the recruitment of mCherry-LC3B to the Shigella septin cage. Scale bar, 1 μm.
Septins and K63 ubiquitin chains are present in separate bacterial microdomains during autophagy of entrapped Shigella
J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.261139