Time-lapse Airyscan confocal movie related to Fig. S6A. Images were acquired using Airyscan fast mode as 12 z-stacks every 5 min. Images were processed using “3D Airyscan processing” using ZEN Black software and max projected. White arrow indicates the interaction between GFP-SEPT6 and mCherry-LC3B mediating the recruitment of mCherry-LC3B to the Shigella septin cage. Scale bar, 1 μm.