Movie 2.

Representative video of dispersion rate of ATP7B from Golgi in response to copper in sub-confluent MDCK cells. MDCK cells transfected with eGFP-ATP7B were plated on glass bottom confocal dishes. Images of sub-confluent MDCK cells treated with 50μM CuCl₂ were captured at an interval of 60 seconds for 1 hour. Pixels having 95% of the signals under basal conditions were considered as Golgi, the disappearance of which represents the Golgi-exit rate.