Movie 3.

Representative video of dispersion rate of ATP7A from Golgi in response to copper in polarized MDCK cells. MDCK cells transfected with mKO2-HA-ATP7A were seeded on 0.4μm, 12mm inserts. Images of polarized MDCK cells treated with 50μM CuCl₂ were captured at an interval of 60 seconds for 1 hour. Pixels having 95% of the signals under basal conditions were considered as Golgi, the disappearance of which represents the Golgi-exit rate.