Movie 1.

Time-lapse microscopy of 3-D motility in 50% Matrigel for the RHΔhx (RH parental 1) and TKO parasites. Left: Videos of projected DIC images (projections of 3-D stack generated by Stack focuser in ImageJ/Fiji). Right: Corresponding processed images with a subset of 3-D trajectories. Time interval: ∼ 1.36 sec. Video speed: 10 frames/s.