Movie 5.

Time-lapse microscopy of A23187 induced-egress for RHΔhx (RH parental) and TKO parasites. A23187 was added at the beginning of the movies to a final concentration of 5 μM. The intracellular wild-type and TKO parasite cultures were placed at 7°C for 1 hr prior to the egress experiment, which was conducted at 37°C. Time interval: 1 sec. Video speed: 60 frames/s. Scale bar: 5 μm.