Movie 1.

BAF-1 dynamics at oocyte- and sperm-derived pronuclei during pronuclear formation, related to Figure 1. Spinning disk confocal fluorescence time series of mNG∧BAF-1 (green) and mCherry:Histone2B (magenta) in fertilized oocyte. Time lapse shows mNG∧BAF-1 enrichment on chromatin transitioning to nuclear membranes during oocyte- (left) and sperm- (right) derived pronuclear formation. Images were acquired every 20 s – playback rate is 80X real time. Scale bar, 5 μm.

Nuclear envelope assembly relies on CHMP-7 in the absence of BAF–LEM-mediated hole closure

Sarah R. Barger, Lauren Penfield, and Shirin Bahmanyar

J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.261385