Movie 2.

BAF-1 is required for LEM-2 enrichment at the sealing plaque following meiosis II, related to Figure 1. Spinning disk confocal fluorescence time series of endogenously tagged LEM-2∧mNG (green) and mCherry:Histone2B (magenta) in fertilized oocytes. Time lapse shows oocytederived pronuclear formation, starting at anaphase II onset, as chromatin moves away from the cortex, and ending with a sealed pronucleus in indicated conditions. LEM-2∧mNG does not accumulate at a discrete focus in the reforming oocyte-derived pronucleus, which undergoes assembly failure. Images were acquired every 20 s – playback rate is 80X real time. Scale bar, 2 μm.