Movie 3.

Reduced and delayed LEM-2∧mNG enrichment at the sealing plaque in baf-1∧L58R embryos, related to Figure 3. Spinning disk confocal fluorescence images of LEM-2∧mNG (green) and mCherry:Histone2B (magenta) in fertilized oocytes in indicated conditions. Time lapse shows oocytederived pronuclear formation, starting at anaphase II onset. Images were acquired every 20 s – playback rate is 80X real time. Scale bar, 2 μm.

Nuclear envelope assembly relies on CHMP-7 in the absence of BAF–LEM-mediated hole closure

Sarah R. Barger, Lauren Penfield, and Shirin Bahmanyar

J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.261385