Movie 5.

Oocyte pronuclear collapse occurs in chmp-7Δ; baf-1∧L58R embryos, related to Figure 5. Spinning disk confocal fluorescence time series of SP12:GFP (ER, green) and mCherry:Histone2B (magenta) marking oocyte-derived pronuclei in indicated conditions. Time lapse shows migration of oocyte- and sperm-derived pronuclei ending in pronuclear meeting approximately at PC regression. Images were acquired every 20 s – playback rate is 80X real time. Scale bar, 5 μm.

Nuclear envelope assembly relies on CHMP-7 in the absence of BAF–LEM-mediated hole closure

Sarah R. Barger, Lauren Penfield, and Shirin Bahmanyar

J Cell Sci 2023. 136:None-None; doi: 10.1242/jcs.261385