Movie 6.

Reduced and delayed CHMP-7 enrichment at the sealing plaque in baf-1∧L58R embryos, related to Figure 5. Spinning disk confocal fluorescence time series of GFP∧CHMP-7 (green) and mCherry:Histone2B (magenta) after anaphase II onset in fertilized oocytes in indicated conditions. Time lapse shows oocyte-derived pronuclear formation, starting at anaphase II onset as chromatin moves away from the cortex and ending with a formed pronucleus in indicated conditions. Images were acquired every 20 s – playback rate is 80X real time. Scale bar, 2 μm.