Movie 7.

Reliance of GFP∧CHMP-7 on the Winged Helix domain of LEM-2 to localize and enrich at the sealing plaque following meiosis II, related to Figure 6. Spinning disk confocal fluorescence time series of GFP∧CHMP-7 (green) and mCherry:Histone2B (magenta) after anaphase II onset in fertilized oocytes in indicated conditions. Left, gray scale images of GFP∧CHMP-7, Right, merged. Time lapse shows oocyte-derived pronuclear formation, starting at anaphase II onset as chromatin moves away from the cortex and ending with a formed pronucleus in lem-2 ΔWH mutant. Left, GFP∧CHMP-7 (grey), Right, merged. Images were acquired every 20 s – playback rate is 80X real time. Scale bar, 2 μm.