Movie 10.
External forces recruit S100A11 to stressed FAs. Using a manual micropipette manipulation setup, external pulling forces were applied onto peripheral FAs in a U2OS cells co-transfected with GFP-S100A11 and vinculin-mCherry. The first image is presented as a merged image (green: GFP-S100A11, red: vinculin-mCherry). After the timelapse series, the first and final frames are again presented as merged imaged.
S100A11 promotes focal adhesion disassembly via myosin II-driven contractility and Piezo1-mediated Ca2+ entry
J Cell Sci 2024. 137:None-None; doi: 10.1242/jcs.261492