Movie 2.

Spatially controlled S100A11 flashes are specific to disassembling FAs. Uncropped (top row) and cropped (lower row) TIRF timelapse image series of a HeLa cell co-expressing GFP-S100A11 (green) and vinculin-mCherry (red) collected at a rate of 1 frame every 4 s. Scale Bar: 5 μm. A distinct S100A11 flash occurs distally to a FA that subsequently disassembles (blue arrow). In contrast, a neighboring FA located about 14 μm away experiences no S100A11 flash and remains stable (yellow arrow).