Movie 2.
Live-cell imaging of U2OS cells transfected with DPF3 siRNA (si DPF3#1). Cells were transfected for 16 hours using calcium phosphate precipitation method and recovered for 8 hours. Live imaging was then started. Images were acquired every 10 min for a total of 24 hours. DPF3-depleted cells spent an unusually long time in mitosis. They did undergo mitosis and showed induction of post-mitotic apoptotic cell death.
Non-canonical role for the BAF complex subunit DPF3 in mitosis and ciliogenesis
J Cell Sci 2024. 137:None-None; doi: 10.1242/jcs.261744