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Movie 1.

Timelapse micrographs of purified human NK cells migrating on ICAM1 or VCAM1 in the presence of IL15. Top panels show stitched fields of view of NK cells from Donor 1 migrating on ICAM1 (left) or VCAM1 (right). Bottom panels show zoomed regions denoted by black boxes for ICAM1 (left) and VCAM1 (right). Images are pseudocolored with yellow denoting cell cytoplasm labelled with Cellbrite Steady 550 and blue denoting nuclei labelled with SPY650-DNA. Micrographs are representative of several fields of view of cells from 3 donors imaged on each surface for 10 hours.

cellPLATO – an unsupervised method for identifying cell behaviour in heterogeneous cell trajectory data

Michael J. Shannon, Shira E. Eisman, Alan R. Lowe, Tyler F. W. Sloan, and Emily M. Mace

J Cell Sci 2024. 137:None-None; doi: 10.1242/jcs.261887