Movie 1.

The movie shows how primary cilia got shortened/lost during the 3 h of a hyperosmotic shock (right) compared to isosmotic culture media (control, left). Rendered images of representative cilia are shown in Fig. 1F. The cells were stably expressing ARL13B-Venus (red) in colorless media. Time stamps indicate the time after adding the hyperosmotic or isoosmotic medium.