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Movie 8.

Actin dynamics in the zygote at entry into mitosis. Z-projection of a confocal timelapse showing the cortical actin. LifeAct::Venus protein was injected into unfertilized oocytes that were subsequently fertilized. The animal pole is up. Note the deformation of the zygote and the transient loss of vegetal cortical actin when the female PN reaches the sperm aster (circa 14 min time point). Scale bar is 40 μm. http://movincell.imevmer.fr/medias/618

Reduction of cortical pulling at mitotic entry facilitates aster centration

Anne Rosfelter, Ghislain de Labbey, Janet Chenevert, Rémi Dumollard, Sebastien Schaub, Zoltan Machaty, Lydia Besnardeau, Daniel Gonzalez Suarez, Céline Hebras, Hervé Turlier, David R. Burgess, and Alex McDougall

J Cell Sci 2024. 137:None-None; doi: 10.1242/jcs.262037