Constitutively active c-Src induces ballooning vasculature in a bead sprouting assay. Corresponds to Fig. 1A. HUVECs transduced with c-Src-mScarlet mutants were grown in a 5 mg/mL fibrin gel bead sprouting assay for 7 days before fixing. Immunofluorescent staining for F-Actin (Phalloidin) and mScarlet signal were used to 3D render sprouts using Imaris software.
Patricia Essebier, Mikaela Keyser, Teodor Yordanov, Brittany Hill, Alexander Yu, Ivar Noordstra, Alpha S. Yap, Samantha J. Stehbens, Anne K. Lagendijk, Lilian Schimmel, and Emma J. Gordon
J Cell Sci
2024. 137:None-None; doi: 10.1242/jcs.262101