Movie 1.
Constitutively active c-Src induces ballooning vasculature in a bead sprouting assay. Corresponds to Fig. 1A. HUVECs transduced with c-Src-mScarlet mutants were grown in a 5 mg/mL fibrin gel bead sprouting assay for 7 days before fixing. Immunofluorescent staining for F-Actin (Phalloidin) and mScarlet signal were used to 3D render sprouts using Imaris software.
c-Src-induced vascular malformations require localised matrix degradation at focal adhesions
J Cell Sci 2024. 137:None-None; doi: 10.1242/jcs.262101