Movie 2.

Constitutively active c-Src induces ballooning vasculature in 3D microvessels. Corresponds to Fig. 1F. HUVECs transduced with mScarlet-tagged c-Src mutants (magenta) grown in PDMS microfluidic vessels containing 2.5 mg/mL collagen matrix for 3 days before fixing. Immunofluorescent staining was performed for VE-Cadherin (green), F-Actin (cyan), and nuclei (DAPI; blue). 3D projection was generated using Imaris software.