Movie 2.

Illustrative movies of sequential render of GLUT4-GFP in ultrathick sections of cleared mouse heart. Sequential movie along the axial imaging depth of a whole mount of optically cleared 3-mm thick mouse ventricular heart section, expressing GLUT4-GFP and with fluorescent intensity colour-coded using the gfireh lookup table. Specimen was imaged using the Mesolens system over a 5 mm × 5 mm × 3 mm volume using a z-step size of 5 μm as described in Method.