Movie 1.

Image stacks represent live microscopy of U2OS cells expressing NMIIB TSMod and Ruby3-Lifeact in Presence of Y27632. From left to right: Ruby3 Lifeact expression in U2OS cells, NMIIB TSMod expression pseudo colored to match CFP/YFP FRET ratio to scale used throughout this manuscript, NMIIB TSMod pseudo colored green and merged with Ruby3 Lifeact. Y27632 treatment starts at 5 minutes to 15 minutes.