Movie 3.

LEL co-traffic with HIV-1 Gag for extended periods of time. Live-cell imaging 2D rendition obtained from recording and stacking several images from the Z-axis in a confocal microscope using THP-1 GagZip macrophages 72 h after HIV-1 induction and incubation with 50 nM Lysotracker red. The movie shows a 30 min recording of a LEL (red) that co-traffics with Gag-GFP (white) during the entire length of the movie. Size bar represents 0.5 μm. The movie was processed and assembled using Imaris v10.0.0.