Movie 4.

HIV-1 Gag-carrying LEL come together at VCCs. Live-cell imaging 2D rendition obtained from recording and stacking several images from the Z-axis in a confocal microscope using THP-1 GagZip macrophages 72 h after HIV-1 induction and incubation with 50 nM Lysotracker red. The movie shows a 15 min recording of a group of LEL (red) that co-traffics with Gag-GFP (white) molecules and deliver them as cargo to already formed Gag-GFP clusters. Size bar represents 1 μm. The movie was processed and assembled using Imaris v10.0.0.