Movie 1.

α-helix is crucial for the decoration of CAMSAP3 at microtubule minus ends. (A) Scheme of the domain organization of CAMSAP3 and the truncated mutants were used to determine the decoration domain of CAMSAP3. (B) Immunostaining for α-tubulin (red) and GFP (green) in HeLa cells transfected with truncation mutants. Scale bar: 10 μm. (C) Signal aggregation quantification of CAMSAP3 mutants was analyzed from experiments shown in (B). n = 30 of each group. Data represent mean ± SEM.