Movie 2.
Control or PPTC7 knockdown cells were seeded in a Nunc glass base 23 mm dishes and pulsed with EGF-647 (green). The cells were treated with the lysosomal dye Lysotracker Red. The cells were imaged in a Zeiss ELYRA super-resolution microscope (63X, NA 1.46). Acquired raw images were subjected to SIM processing and videos were exported and edited. The localization of EGF and lysosomes in whole cells is shown.
Mitochondrial phosphatase PPTC7 promotes EGFR recycling by facilitating VPS4A endosomal localization
J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.263676