Movie 1.

ERMES foci move with the mitochondrial network. Cells expressing the mitochondrial matrix marker MitoRed, Mdm12-GFP, and Num1-Halo were grown to mid-log phase, labeled with JFX650, adhered to a ConA treated confocal dish, and imaged. The movie is a max projection of a full Z-stack. Merged fluorescence channels are shown on top and the individual fluorescence channels from the Mdm12-GFP and Num1-Halo channels are shown in gray scale below. All channels are overlayed with a bright field image. The trajectories of the Num1 and Mdm12 foci were identified using the TrackMate plugin for Fiji and are indicated by a colored line overlayed on the grayscale images. Stills from this movie are shown in Fig. 2A. Scale bar 2 μm. Time is in min:s.