Loss of MECA and changes in growth conditions modulate the rates of ERMES dynamics. Cells expressing Mdm12-GFP and MitoRed in wild-type or num1Δ backgrounds were grown in SCD or SCEG to mid-log phase, adhered to ConA treated confocal dishes, and imaged. Representative examples of each genetic background and growth condition are tiled together to aid visual comparison. A merged fluorescence channel is shown on top and the individual Mdm12-GFP channel is shown in grayscale below. Fluorescence channels are overlayed with a bright field image. All movies are max projections of full Z-stacks. Images from
this movie are shown in Fig. 4A-D. Scale bar 2 μm. Time is in min:s.