Movie 4.

ERMES foci divide. Cells were grown and imaged identically to Movie 2. The movie depicts an event where one distinct Mdm12 focus divides into two distinct foci. The movie is an individual slice from a full Z-stack to demonstrate the fission event is happening on the same imaging plane. A merged image of all fluorescence channels is shown on top and the Mdm12-Halo channel is shown in grayscale below. Images from this movie are shown in Fig. 3C. Scale bar 2 μm. Time is in min:s.