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Movie 2.

Para-nitro Blebbistatin treatment minimally perturb NJCCs. Live-cell spinning disk confocal microscopy of Lifeact-Emerald stable cell line mESC in 2iLIF showed that NJCCs appear to be largely unaffected by treatment with 10 μM Para-nitro-Blebbistatin. Time-lapse movie was taken with 1min interval. Scale bar: 5 μm.

Ground-state pluripotent stem cells are characterized by Rac1-dependent cadherin-enriched F-actin complexes

Shiying Liu, Yue Meng, Xi Lan, Rong Li, and Pakorn Kanchanawong

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.263811