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Movie 4.

Three-dimensional reconstruction of mitochondria in A549 RFFL-EGFP stable cells. Cells were immunostained with an anti-TOMM20 antibody, and imaging was done with confocal microscopy. 3-D reconstruction (Volume) of the images was done from deconvoluted Z stacks. The contrast was adjusted for better visibility. Related to Fig. 1A.

Endosomal RFFL ubiquitin ligase regulates mitochondrial morphology by targeting mitofusin 2

Nikhil Dev Narendradev, Rishith Ravindran, Parul Jain, Shikha Chaudhary, Anoop Kumar G. Velikkakath, Abyasree Sudharman, Adithya Janardhanan, Tapas Chandra Nag, Subhash Chandra Yadav, and Srinivasa Murty Srinivasula

J Cell Sci 2025. 138:None-None; doi: 10.1242/jcs.263830