Movie 1.

Relocalization of ATG9A-FKBP-mCherry causes co-relocation of endogenous GFP-TPD54. Movie of GFP-TPD54 (panel 2, green) knock-in cells expressing ATG9A-FKBP-mCherry (panel 1, red) and MitoTrap (panel 3, blue); capture of ATG9Apositive vesicles at the mitochondria is induced by rapalog (5 μM at 40 s). Time, mm:ss. Playback, 10 fps. Scale bar, 10 μm.