Movie 1

A Confocal microscope Z-stack spanning the depth of the interface of a Stage 6 barnacle stained with SYPRO Ruby for protein (in red/pink) and Pro-Q Diamond for phosphoprotein (in yellow/orange). The autofluorescence of the animal excited with 405 nm light (in blue), the scale bar is 50 µm.

Barnacle biology before, during and after settlement and metamorphosis: a study of the interface

Tara Essock-Burns, Neeraj V. Gohad, Beatriz Orihuela, Andrew S. Mount, Christopher M. Spillmann, Kathryn J. Wahl, and Daniel Rittschof

Journal of Experimental Biology 2017. 220:194-207; doi: 10.1242/jeb.145094