To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie 3 Tissue Envelope at 96 hours post isolation (hpi) showing the accumulation of detached vacuolated digestive cells at the blind-end of an isolated endodermal canal. Establishing and maintaining primary cell cultures derived from the ctenophore Mnemiopsis leidyi Lauren E. Vandepas, Kaitlyn J. Warren, Chris T. Amemiya, and William E. Browne Journal of Experimental Biology 2017. 220:1197-1201; doi: 10.1242/jeb.152371 Download Original Video