Double circle assay
The movie demonstrates the double circle assay for 30 min at 90× playback speed: when the outer circle was 1.0% agar and the inner circle was 1.0% agar (left); and when the outer circle was 1.0% agar and the inner circle was 0.5% agar (right). The larvae return to the inner circle in the situation shown on the right. The time courses of P.I. values during the first 5 min were shown in Fig. S1B.