Movie 2

Predatory hunting of virtual prey in a starved praying mantis before and after insulin injection.
This video shows two clips representative of the behavioral changes in the praying mantis after insulin injection while viewing virtual pray. The first clip shows the starved pre-injection baseline where a praying mantis was shown a total of 40 appearances of a moving black ellipse. The first prey presentation (12.5 cm away, 225°, 0:01) evoked attention followed by stepping and repeated striking at the stimulus. The animal also shows stepping and striking behavior towards the second presentation (10 cm away, 0°, 0:16). The second clip shows the same praying mantis 15 minutes after injection of 0.05 ml of 200 μg/ml insulin. Here, the animal does not attend to the frontal prey stimulus (5 cm away, 180°, 0:26) shown in the back right of the animal but does attend and strike at the next stimulus (5 cm away, 0°, 0:38) after taking one step in approach.