Movie 3

Thermogenetic activation of VT041723-GAL4 neurons induces regurgitation after ingestion. Regurgitation observed upon placing a VT041723-GAL4>dTrpA1 female fly pre-fed with 0.5 μL of 100 mM sucrose on a 31 °C heated block. Note that the fly held the proboscis with a bubble at the tip for the whole 7-minute recording time.