Movie 2

Development of tracheal and gastrointestinal system. Movies of (top) first and (bottom) second worker honey specimens show a time sequence of the volume-rendered images in sagittal view of the segmented organs: gastrointestinal tract is shown in red, honey stomach in orange and tracheal system in blue. In the movies, curvature development of the gastrointestinal tract is clearly visible. The tract can be reduced to a single narrow curve spanning through the entire tract region. The curve enabled measurement of the tract length time dependence as well as the measurement of the distance between the tract end points. This information was needed for the calculation of the tract's tortuosity and its time dependence during the metamorphosis that is shown in Fig 2.