Movie 1

3D projection of developing scales in a clear wing region 48 hours after pupal formation.
3D projection and rotation of the same scales shown in Fig. 2F, 48 hours APF in a clear wing region. WGA (magenta) stains cell membranes and phalloidin (green) stains F-actin and DAPI (blue) stains nuclei. Short actin filaments have reorganized and formed smaller numbers of thick, regularly spaced parallel bundles just under the surface of the cell membrane. Scales alternate with future forked scales appearing as triangular shapes and longer future bristle-like shapes.