Movie 1

Example tube foot motility assay recording. To assess the motility of tube feet, each sea urchin was immersed in ASW in a circular tank atop of a printed template comprised of a circle divided into 16 equally-sized segments. One-minute recordings were obtained from directly above the sea urchin. Quantification of motility was achieved counting the total number of times any tube foot crossed any line segment (see Methods for details).