Movie 1.

Long-term live imaging of a honeybee embryo injected with mRNA encoding mEmerald-labeled anti-actin nanobodies. Embryogenesis is shown along the lateral side (illumination ventral) from 00:00–56:00 h with an interval of 0:30 h between the time points. The video is based on DS0001. Frame rate is five frames per second.

Long-term fluorescence live imaging of honeybee embryos using light sheet fluorescence microscopy and halocarbon-based liquids

Oksana Netschitailo, Paul Siefert, Markus M. Middeke, Artemiy Golden, Christin Schrod, Tim Beringer, Bernd Grünewald, Ernst H. K. Stelzer, and Frederic Strobl

Biology Open 2025. 14:None-None; doi: 10.1242/bio.062151