Movie 1

Co-migration of CVM cells and PGCs
Embryo expressing the HC3 reporter and vasa-GFP imaged live from the beginning of CVM migration, through germ band retraction. Time is displayed as hours: minutes. At the beginning, the migrating CVM cells have just begun to separate into two bilateral clusters and move forward while the germ cells are emerging from the pmg. By 1.5 hours, the CVM start to overtake the germ cells. At 50 minutes, a germ cell can be seen squeezing between CVM cells, as the germ cells are moving laterally. About half of the CVM cells have passed the “turn” when germ band retraction begins at ~3 hr. At this time the germ cells completely trail the CVM, and are present as two distinct bilateral groups that move toward the somatic gonadal precursors. Genotype of embryo is homozygous for the HC3, vasa-GFP reporter chromosome, and is in a wild-type background. Contrast was optimized with ImageJ to better visualize each cell population. First movie is two color showing CVM and PGCs; second movie is the same embryos showing CVM cell migration only for comparison.