To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Play VideoPlayMuteCurrent Time 0:00/Duration Time 0:00Loaded: 0%Progress: 0%Stream TypeLIVERemaining Time -0:00 Playback Rate1ChaptersChaptersdescriptions off, selectedDescriptionssubtitles off, selectedSubtitlescaptions off, selectedCaptionsAudio TrackFullscreenThis is a modal window. Captions Settings Dialog Beginning of dialog window. Escape will cancel and close the window. Text Color White Black Red Green Blue Yellow Magenta Cyan Transparency Opaque Semi-Opaque Background Color Black White Red Green Blue Yellow Magenta Cyan Transparency Opaque Semi-Transparent Transparent Window Color Black White Red Green Blue Yellow Magenta Cyan Transparency Transparent Semi-Transparent Opaque Font Size 50% 75% 100% 125% 150% 175% 200% 300% 400% Text Edge Style None Raised Depressed Uniform Dropshadow Font Family Proportional Sans-Serif Monospace Sans-Serif Proportional Serif Monospace Serif Casual Script Small Caps Defaults Done Movie 1 Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes Alexa Burger, Helen Lindsay, Anastasia Felker, Christopher Hess, Carolin Anders, Elena Chiavacci, Jonas Zaugg, Lukas M. Weber, Raul Catena, Martin Jinek, Mark D. Robinson, and Christian Mosimann Development 2016. 143:2025-2037; doi: 10.1242/dev.134809 Download Original Video