Movie 1

Light sheet composite volumetric image of the telencephalon and diencephalon of an E16 chick embryo brain from its right lateral edge to the midline. An E16 embryo was perfused, fixed with 4% PFA, and the brain was dissected out. The isolated brain was CUBIC-cleared, and immunostained with anti-orexin (green) and anti-c-Fos (red) antibodies. Nuclei were counterstained with DAPI (blue). Sagittal images were acquired with a 2x objective. Sagittal stack animation, rostral is to the left, dorsal is up.