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Movie 5

Higher-magnification volumetric image of a portion of the same E4 chick embryo seen in Supplementary movie 4. A 10x objective was used to acquire movies of the whole embryo. The movie is a sagittal stack animation in the same orientation as in Supplementary movie 4.

Optimized CUBIC protocol for three-dimensional imaging of chicken embryos at single-cell resolution

María Victoria Gómez-Gaviro, Evan Balaban, Diana Bocancea, María Teresa Lorrio, Maria Pompeiano, Manuel Desco, Jorge Ripoll, and Juan José Vaquero

Development 2017. 144:2092-2097; doi: 10.1242/dev.145805