Movie 1.

Spinning disk time-lapse imaging of a pupa with and without immersion in PBS. A 12 hAPF pupa expressing Ecad:GFP was mounted under a coverslip and imaged by spinning disk microscope for 50 min before adding PBS (t=0). Cell divisions can be observed throughout the tissue before PBS addition. Cells in metaphase at t=0 min do not complete mitosis (arrowheads). The acquisition parameters are detailed in Table S1. Scale bar: 50 μm.