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Movie 1.

Light-sheet microscopy time-lapse video of zebrafish epiboly. Exemplary optical sections from a Tg(bactin:hRas-EGFP) embryo at 25 time points acquired from 5 hpf (≈40% epiboly) to 9 hpf (≈90% epiboly), respectively, following image acquisition using a light-sheet fluorescence microscope and multi-view reconstruction described in Section 4.4. hRas-EGFP labels the cell membranes (green). ECS is marked by TMR-Dextran injection (orange).

Morphogen gradients are regulated by porous media characteristics of the developing tissue

Justina Stark, Rohit Krishnan Harish, Ivo F. Sbalzarini, and Michael Brand

Development 2025. 152:None-None; doi: 10.1242/dev.204312