Movie 5.

T cells migrate with a cytoplasmic leading edge lacking f-actin. A representative movie of a T cell migrating with F-actin poor and cytoplasmic leading edges in wounded scale explants from tg(lck:GFP; lck:lifeact-ruby). In the top frame, lifeact-ruby is pseudo colored as a heatmap. In the bottom frame, the GFP (green) and lifeact-ruby (magenta) channels are merged. Time = mm:ss. Scae bar = 10 μm.